Effects of melatonin on 2-deoxy- 1-14C glucose uptake within rat suprachiasmatic nucleus.

TitleEffects of melatonin on 2-deoxy- 1-14C glucose uptake within rat suprachiasmatic nucleus.
Publication TypeJournal Article
Year of Publication1988
JournalThe American journal of physiology
Volume255
Issue2 Pt 2
PaginationR332-7
ISSN0002-9513
Abstract

Previously, we have demonstrated that metabolic activity, shown by autoradiographic determination of 2-deoxy-[1-14C]glucose (2-DG) uptake, within the rat hypothalamic suprachiasmatic nuclei (SCN) was inhibited by subcutaneous injection of 1 mg/kg melatonin. To determine whether this effect was specific to a particular time of day, the effects of melatonin on 2-DG uptake were studied in several hypothalamic areas, including the SCN, supraoptic nuclei (SON), lateral hypothalamic area (LHA), and anterior hypothalamic area (AHA) every 4 h throughout the circadian day. In a second experiment, the effects of different melatonin doses were studied at the time of day at which melatonin had its maximal effect to determine the dose-response relationship of melatonin-induced inhibition of SCN 2-DG uptake. The data indicate that melatonin inhibited 2-DG uptake in the SCN alone at one time of day, primarily at circadian time (CT) 6 and CT10, 2-6 h before subjective dusk, and secondarily at CT22, just before subjective dawn. This effect was dose dependent with a 50% effective dose of 1.49 +/- 2.30 micrograms/kg. The temporal and dose-response characteristics of these effects are similar to those characterizing the entraining effects of melatonin on circadian patterns of locomotion and drinking.

URLhttp://www.physiology.org/doi/abs/10.1152/ajpregu.1988.255.2.R332?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
DOI10.1152/ajpregu.1988.255.2.R332
Short TitleAm J Physiol
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