High-quality combinatorial protein libraries using the binary patterning approach.

TitleHigh-quality combinatorial protein libraries using the binary patterning approach.
Publication TypeJournal Article
JournalMethods in molecular biology (Clifton, N.J.)
Date Published2014

Protein combinatorial libraries have become a platform technology for exploring protein sequence space for novel molecules for use in research, synthetic biology, biotechnology, and medicine. To expedite the isolation of proteins with novel/desired functions using screens and selections, high-quality approaches that generate protein libraries rich in folded and soluble structures are desirable for this goal. The binary patterning approach is a protein library design method that incorporates elements of both rational design and combinatorial diversity to specify the arrangement of polar and nonpolar amino acid residues in the context of a desired, folded tertiary structure template. An overview of the considerations necessary to design and construct binary patterned libraries of de novo and natural proteins is presented.

Short TitleMethods Mol Biol
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