Physical maps of the genomes of three Bacillus cereus strains.

TitlePhysical maps of the genomes of three Bacillus cereus strains.
Publication TypeJournal Article
Year of Publication1992
JournalJournal of bacteriology
Volume174
Issue11
Pagination3750-6
ISSN0021-9193
Abstract

NotI restriction maps of the chromosomes from Bacillus cereus ATCC 10876, ATCC 11778, and the B. cereus type strain ATCC 14579 have been established and compared with the previously established map of B. cereus ATCC 10987. Between 10 and 14 NotI fragments were observed, ranging from 15 to 1,300 kb, in digests of DNA from the various strains. The sizes of the genomes varied between 5.4 and 6.3 Mb. The maps were constructed by hybridization of 42 random probes, prepared from B. cereus ATCC 10987 libraries, to fragments from partial and complete NotI digests, separated by pulsed-field gel electrophoresis. Nine probes were specific for ATCC 10987 only. Probes for five B. subtilis and five B. cereus genes were also used. The NotI restriction fragment patterns of the four strains were strikingly different.

URLhttp://jb.asm.org/cgi/pmidlookup?view=long&pmid=1592826
Short TitleJ Bacteriol
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