Chris Richards

Education:
Ph.D. Georgia Institute of Technology 2005-2009
Postdoctoral Fellow California Institute of Technology 2009-2012
Biography:

We are an interdisciplinary group that integrates nanotechnology, neuroscience, and biophysics.

For more information, please visit the Richards Group website.

Research Interests:
Biophysics
Membrane receptors
Single Molecule Imaging
Plasmon Enhanced Fluorescence
Research

We are an interdisciplinary group that integrates nanotechnology, neuroscience, and biophysical chemistry.  A major component of our research is the development of single-molecule fluorescence imaging techniques that allow us to isolate individual membrane receptors. These studies primarily focus on the interaction of fluorophores with plasmonic metal structures to enhance the emission from fluorescently labeled proteins.  We use these techniques to understand how modifications in membrane receptor trafficking and assembly alters neuronal function and is involved in the mechanisms of neurodegenerative diseases and addiction.

Graduate Training

Biophysical Chemistry Neuroscience

PubMed Publications*: 
  • SP Aryal; M Xia; PI Ortinski; CI Richards"Study of Calcium Signaling in Astrocytes with a Novel Endoplasmic Reticulum-Targeted GCaMP Sensor."Current protocols2.8(2022):e491.Details. Full text
  • TC Messina; BR Srijanto; CP Collier; Kravchenko II; CI Richards"Gold Ion Beam Milled Gold Zero-Mode Waveguides."Nanomaterials (Basel, Switzerland)12.10(2022):Details. Full text
  • SP Aryal; M Xia; E Adindu; C Davis; PI Ortinski; CI Richards"ER-GCaMP6f: An Endoplasmic Reticulum-Targeted Genetic Probe to Measure Calcium Activity in Astrocytic Processes."Analytical chemistry94.4(2022):2099-2108.Details. Full text
  • SP Aryal; X Fu; JN Sandin; KR Neupane; JE Lakes; ME Grady; CI Richards"Nicotine induces morphological and functional changes in astrocytes via nicotinic receptor activity."Glia69.8(2021):2037-2053.Details. Full text
  • SP Aryal; KR Neupane; AA Masud; CI Richards"Characterization of Astrocyte Morphology and Function Using a Fast and Reliable Tissue Clearing Technique."Current protocols1.10(2021):e279.Details. Full text
  • H Peng; BT Harvey; CI Richards; K Nixon"Neuron-Derived Extracellular Vesicles Modulate Microglia Activation and Function."Biology10.10(2021):Details. Full text
  • X Fu; P Sompol; JA Brandon; CM Norris; T Wilkop; LA Johnson; CI Richards"<i>In Vivo</i> Single-Molecule Detection of Nanoparticles for Multiphoton Fluorescence Correlation Spectroscopy to Quantify Cerebral Blood Flow."Nano letters20.8(2020):6135-6141.Details. Full text
  • X Fu; Y Song; A Masud; K Nuti; JE DeRouchey; CI Richards"High-throughput fluorescence correlation spectroscopy enables analysis of surface components of cell-derived vesicles."Analytical and bioanalytical chemistry412.11(2020):2589-2597.Details. Full text
  • JE Lakes; CI Richards; MD Flythe"Inhibition of Bacteroidetes and Firmicutes by select phytochemicals."Anaerobe61.(2020):102145.Details. Full text
  • X Fu; FH Moonschi; AM Fox-Loe; AA Snell; DM Hopkins; AJ Avelar; BJ Henderson; Pauly JR; CI Richards"Brain Region Specific Single-Molecule Fluorescence Imaging."Analytical chemistry91.15(2019):10125-10131.Details. Full text
  • AA Snell; KR Neupane; McCorkle JR; X Fu; FH Moonschi; EB Caudill; J Kolesar; CI Richards"Cell-Derived Vesicles for in Vitro and in Vivo Targeted Therapeutic Delivery."ACS omega4.7(2019):12657-12664.Details. Full text
  • J Chen; S Wang; Z Zhang; CI Richards; R Xu"Heat shock protein 47 (HSP47) binds to discoidin domain-containing receptor 2 (DDR2) and regulates its protein stability."The Journal of biological chemistry294.45(2019):16846-16854.Details. Full text
  • FH Moonschi; CB Hughes; GM Mussman; JL Fowlkes; CI Richards; I Popescu"Advances in micro- and nanotechnologies for the GLP-1-based therapy and imaging of pancreatic beta-cells."Acta diabetologica55.5(2018):405-418.Details. Full text
  • FH Moonschi; AM Fox-Loe; X Fu; CI Richards"Mammalian Cell-derived Vesicles for the Isolation of Organelle Specific Transmembrane Proteins to Conduct Single Molecule Studies."Bio-protocol8.9(2018):Details. Full text
  • AM Fox-Loe; FH Moonschi; CI Richards"Organelle-specific single-molecule imaging of α4β2 nicotinic receptors reveals the effect of nicotine on receptor assembly and cell-surface trafficking."The Journal of biological chemistry292.51(2017):21159-21169.Details. Full text
  • WE Martin; N Ge; BR Srijanto; E Furnish; CP Collier; CA Trinkle; CI Richards"Real-Time Sensing of Single-Ligand Delivery with Nanoaperture-Integrated Microfluidic Devices."ACS omega2.7(2017):3858-3867.Details. Full text
  • AM Fox-Loe; BJ Henderson; CI Richards"Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking."Journal of visualized experiments : JoVE121(2017):Details. Full text
  • Z Zhang; MM Baksh; MG Finn; DK Heidary; CI Richards"Direct Measurement of Trafficking of the Cystic Fibrosis Transmembrane Conductance Regulator to the Cell Surface and Binding to a Chemical Chaperone."Biochemistry56.1(2017):240-249.Details. Full text
  • DK Heidary; A Fox; CI Richards; EC Glazer"A High-Throughput Screening Assay Using a Photoconvertable Protein for Identifying Inhibitors of Transcription, Translation, or Proteasomal Degradation."SLAS discovery : advancing life sciences R & D22.4(2017):399-407.Details. Full text
  • WE Martin; BR Srijanto; CP Collier; T Vosch; CI Richards"A Comparison of Single-Molecule Emission in Aluminum and Gold Zero-Mode Waveguides."The journal of physical chemistry. A120.34(2016):6719-27.Details. Full text
  • AM Fox; FH Moonschi; CI Richards"The nicotine metabolite, cotinine, alters the assembly and trafficking of a subset of nicotinic acetylcholine receptors."The Journal of biological chemistry290.40(2015):24403-12.Details. Full text
  • JS Wieskopf; J Mathur; W Limapichat; MR Post; M Al-Qazzaz; RE Sorge; LJ Martin; DV Zaykin; SB Smith; K Freitas; JS Austin; F Dai; J Zhang; J Marcovitz; AH Tuttle; PM Slepian; S Clarke; RM Drenan; J Janes; Al Sharari; SK Segall; EK Aasvang; W Lai; R Bittner; CI Richards; GD Slade; H Kehlet; J Walker; U Maskos; JP Changeux; M Devor; W Maixner; L Diatchenko; I Belfer; DA Dougherty; AI Su; SC Lummis; Imad Damaj; HA Lester; A Patapoutian; JS Mogil"The nicotinic α6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors."Science translational medicine7.287(2015):287ra72.Details. Full text
  • FH Moonschi; AK Effinger; X Zhang; WE Martin; AM Fox; DK Heidary; JE DeRouchey; CI Richards"Cell-derived vesicles for single-molecule imaging of membrane proteins."Angewandte Chemie (International ed. in English)54.2(2015):481-4.Details. Full text
  • R Srinivasan; BJ Henderson; HA Lester; CI Richards"Pharmacological chaperoning of nAChRs: a therapeutic target for Parkinson's disease."Pharmacological research83.(2014):20-9.Details. Full text
  • WA Nichols; BJ Henderson; C Yu; RL Parker; CI Richards; HA Lester; JM Miwa"Lynx1 shifts α4β2 nicotinic receptor subunit stoichiometry by affecting assembly in the endoplasmic reticulum."The Journal of biological chemistry289.45(2014):31423-32.Details. Full text
  • SK Das; Y Liu; S Yeom; DY Kim; CI Richards"Single-particle fluorescence intensity fluctuations of carbon nanodots."Nano letters14.2(2014):620-5.Details. Full text
  • KA Murach; Vechetti IJ Jr; DW Van Pelt; SE Crow; CM Dungan; VC Figueiredo; K Kosmac; X Fu; CI Richards; CS Fry; JJ McCarthy; CA Peterson"Fusion-Independent Satellite Cell Communication to Muscle Fibers During Load-Induced Hypertrophy."Function (Oxford, England)1.1(0):zqaa009.Details.
  • Z Zhang; DK Heidary; CI Richards"High resolution measurement of membrane receptor endocytosis."Journal of biological methods5.4(0):e105.Details.
* Publications are automatically pulled from pubmed.gov based on a user-specific query. Results may include incorrect citations. See: Tutorial on improving PubMed results.