The French press: a repeatable and high-throughput approach to exercising zebrafish (<i>Danio rerio</i>).
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Abstract |
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Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34-0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes. |
Year of Publication |
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0
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Journal |
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PeerJ
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Volume |
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6
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Number of Pages |
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e4292
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Date Published |
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2018
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DOI |
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10.7717/peerj.4292
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Short Title |
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PeerJ
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